Southern Terminus: Springer Mountain, Georgia
U.S. Highway 76 in northeast Georgia
U.S. Highway 64 near Nantahala River, North Carolina
U.S. Highway 19/U.S. Highway 74 near Fontana Lake, North Carolina
U.S. Highway 441 at North Carolina/Tennessee border
Interstate 40 at North Carolina/Tennessee border
U.S. Highway 25/U.S. Highway 70 in Hot Springs, North Carolina
Interstate 26/U.S. Highway 23 at North Carolina/Tennessee border
U.S. Highway 19W at North Carolina/Tennessee border
U.S. Highway 19E near Roan Mountain, Tennessee
U.S. Highway 321 at Watauga Lake, Tennessee
U.S. Highway 421 near South Holston Lake, Tennessee
U.S. Highway 58 in Damascus, Virginia
Interstate 81 near Marion, Virginia
Interstate 77/U.S. Highway 52 near Wytheville, Virginia
U.S. Highway 460 in Pearisburg, Virginia- Interstate 81 near Roanoke, Virginia
U.S. Highway 501 at James River, Virginia
U.S. Highway 60 near Buena Vista, Virginia
Interstate 64 near Waynesboro, Virginia
U.S. Highway 250 near Waynesboro, Virginia
U.S. Highway 33 in Shenandoah National Park
U.S. Highway 211 near Luray, Virginia
U.S. Highway 522 near Front Royal, Virginia
Interstate 66 in Front Royal, Virginia
U.S. Highway 17/U.S. Highway 50 near Waterloo, Virginia
U.S. Highway 340 in Harpers Ferry, West Virginia- U.S. Highway 340 in Sandy Hook, Maryland
Interstate 70/U.S. Highway 40 near Hagerstown, Maryland
U.S. Highway 30 near Fayetteville, Pennsylvania
Interstate 76 near Middlesex Township, Pennsylvania
U.S. Highway 11 near Middlesex Township, Pennsylvania- Interstate 81 near Middlesex Township, Pennsylvania
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U.S. Highway 11/U.S. Highway 15 in Duncannon, Pennsylvania 
U.S. Highway 22/U.S. Highway 322 in Duncannon, Pennsylvania- Interstate 81 near Fort Indiantown Gap, Pennsylvania
Interstate 476 near Slatington, Pennsylvania
Interstate 80 at Pennsylvania/New Jersey border
U.S. Highway 206 near Frankford, New Jersey
Interstate 87 near Harriman, New York
U.S. Highway 6/U.S. Highway 9W/U.S. Highway 202 near Fort Montgomery, New York
U.S. Highway 9 near Garrison, New York
Taconic State Parkway near Shenandoah, New York
Interstate 84 near Whaley Lake, New York
U.S. Highway 7 near Falls Village, Connecticut
U.S. Highway 44 near Salisbury, Connecticut- U.S. Highway 7 near Great Barrington, Massachusetts
Interstate 90 near Lee, Massachusetts
U.S. Highway 20 near Lee, Massachusetts
U.S. Highway 4 near Rutland, Vermont
Interstate 89 near West Hartford, Vermont
U.S. Highway 5 near Hanover, New Hampshire (US-5 is in Vermont)
Interstate 91 near Hanover, New Hampshire (I-91 is in Vermont)
Interstate 93/U.S. Highway 3 near Franconia, New Hampshire
U.S. Highway 302 in White Mountain National Forest
U.S. Highway 2 near Berlin, New Hampshire
U.S. Highway 201 in Caratunk, Maine
U.S. Highway 16 in Monson, Maine
Northern terminus: Mt. Katahdin, Maine
